Induction and repression effects on CYP and transporter protein abundance by azole mixture uptake in rat liver.

ABSTRACT: Detection of mixture effects is a major challenge in current experimental and regulatory toxicology. Robust markers are needed that are easy to quantify and responsive to chemical stressors in a broad dose range. Several hepatic enzymes and proteins related to drug metabolism like cytochrome-P-450 (CYP) enzymes and transporters have been shown to be responsive to pesticide active substances in a broad dose range and are therefore good candidates to be used as markers for mixture toxicity. Even though they can be well quantified at the mRNA level, quantification on the protein level is challenging because most of these proteins are membrane bound. Here we report the development of mass spectrometry-based assays using triple-x-proteomics (TXP) antibodies in combination with targeted selected

SUBMITTER: Hammer H 

PROVIDER: S-EPMC7744963 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature