Influence of fluorophore and linker length on the localization and trafficking of fluorescent sterol probes.

ABSTRACT: Fluorescent sterol probes, comprising a fluorophore connected to a sterol backbone by means of a linker, are promising tools for enabling high-resolution imaging of intracellular cholesterol. In this study, we evaluated how the size of the linker, site of its attachment and nature of the fluorophore, affect the localization and trafficking properties of fluorescent sterol probes. Varying lengths of linker using the same fluorophore affected cell penetration and retention in specific cell compartments. A C-4 linker was confirmed as optimal. Derivatives of heterocyclic sterol precursors attached with identical C-4 linker to different fluorophores at diverse positions also showed significant differences in their binding properties to various intracellular compartments and kinetics of traffick

SUBMITTER: Kralova J 

PROVIDER: S-EPMC7745015 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature