Selectively enhancing radiosensitivity of cancer cells via in situ enzyme-instructed peptide self-assembly.

ABSTRACT: The radiotherapy modulators used in clinic have disadvantages of high toxicity and low selectivity. For the first time, we used the in situ enzyme-instructed self-assembly (EISA) of a peptide derivative (Nap-G^DF^DFpYSV) to selectively enhance the sensitivity of cancer cells with high alkaline phosphatase (ALP) expression to ionizing radiation (IR). Compared with the in vitro pre-assembled control formed by the same molecule, assemblies formed by in situ EISA in cells greatly sensitized the ALP-high-expressing cancer cells to γ-rays, with a remarkable sensitizer enhancement ratio. Our results indicated that the enhancement was a result of fixing DNA damage, arresting cell cycles and inducing cell apoptosis. Interestingly, in vitro pre-

SUBMITTER: Gao Y 

PROVIDER: S-EPMC7745053 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature