The protective role of intracellular glutathione in Saccharomyces cerevisiae during lignocellulosic ethanol production.

ABSTRACT: To enhance the competitiveness of industrial lignocellulose ethanol production, robust enzymes and cell factories are vital. Lignocellulose derived streams contain a cocktail of inhibitors that drain the cell of its redox power and ATP, leading to a decrease in overall ethanol productivity. Many studies have attempted to address this issue, and we have shown that increasing the glutathione (GSH) content in yeasts confers tolerance towards lignocellulose inhibitors, subsequently increasing the ethanol titres. However, GSH levels in yeast are limited by feedback inhibition of GSH biosynthesis. Multidomain and dual functional enzymes exist in several bacterial genera and they catalyse the GSH biosynthesis in a single step without the feedback inhibition. To test if even higher intracellular g

SUBMITTER: Raghavendran V 

PROVIDER: S-EPMC7745091 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature