Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field.

ABSTRACT: Photorespiration is required in C₃ plants to metabolize toxic glycolate formed when ribulose-1,5-bisphosphate carboxylase-oxygenase oxygenates rather than carboxylates ribulose-1,5-bisphosphate. Depending on growing temperatures, photorespiration can reduce yields by 20 to 50% in C₃ crops. Inspired by earlier work, we installed into tobacco chloroplasts synthetic glycolate metabolic pathways that are thought to be more efficient than the native pathway. Flux through the synthetic pathways was maximized by inhibiting glycolate export from the chloroplast. The synthetic pathways tested improved photosynthetic quantum yield by 20%. Numerous homozygous transgenic lines increased biomass productivity by >40% in replicated field trials. These results show that engineering a

SUBMITTER: South PF 

PROVIDER: S-EPMC7745124 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature