Nanomolar Protein-Protein Interaction Monitoring with a Label-Free Protein-Probe Technique.

ABSTRACT: Protein-protein interactions (PPIs) are an essential part of correct cellular functionality, making them increasingly interesting drug targets. While Förster resonance energy transfer-based methods have traditionally been widely used for PPI studies, label-free techniques have recently drawn significant attention. These methods are ideal for studying PPIs, most importantly as there is no need for labeling of either interaction partner, reducing potential interferences and overall costs. Already, several different label-free methods are available, such as differential scanning calorimetry and surface plasmon resonance, but these biophysical methods suffer from low to medium throughput, which reduces suitability for high-throughput screening (HTS) of PPI inhibitors. Differential scanning flu

SUBMITTER: Valtonen S 

PROVIDER: S-EPMC7745204 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature