Characterisation of gene and pathway expression in stabilised blood from children with coeliac disease.

ABSTRACT:

Introduction

A coeliac disease (CD) diagnosis is likely in children with levels of tissue transglutaminase autoantibodies (anti-TG2) >10 times the upper reference value, whereas children with lower anti-TG2 levels need an intestinal biopsy to confirm or rule out CD. A blood sample is easier to obtain than an intestinal biopsy sample, and stabilised blood is suitable for routine diagnostics because transcript levels are preserved at sampling. Therefore, we investigated gene expression in stabilised whole blood to explore the possibility of gene expression-based diagnostics for the diagnosis and follow-up of CD.

Design

We performed RNA sequencing of stabilised whole blood from active CD cases (n=10), non-CD cases (n=10), and treated CD cases on a gluten-free diet (n=10) to ide

SUBMITTER: Bragde HG 

PROVIDER: S-EPMC7745333 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature