Spider venom components decrease glioblastoma cell migration and invasion through RhoA-ROCK and Na⁺/K⁺-ATPase β2: potential molecular entities to treat invasive brain cancer.

ABSTRACT:

Background

Glioblastoma (GB) cells have the ability to migrate and infiltrate the normal parenchyma, leading to the formation of recurrent tumors often adjacent to the surgical extraction site. We recently showed that Phoneutria nigriventer spider venom (PnV) has anticancer effects mainly on the migration of human GB cell lines (NG97 and U-251). The present work aimed to investigate the effects of isolated components from the venom on migration, invasiveness, morphology and adhesion of GB cells, also evaluating RhoA-ROCK signaling and Na⁺/K⁺-ATPase β2 (AMOG) involvement.

Methods

Human (NG97) GB cells were treated with twelve subfractions (SFs-obtained by HPLC from PnV). Migration and invasion were evaluated by scratch wound healing and transwell assays,

SUBMITTER: Barreto N 

PROVIDER: S-EPMC7745393 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature