Stability of canine and feline cerebrospinal fluid samples regarding total cell count and cell populations stored in "TransFix®/EDTA CSF sample storage tubes".

ABSTRACT:

Background

Because of fast leucocyte degeneration in cerebrospinal fluid (CSF) laboratory examinations of CSF samples should be performed approximately within 30 min after withdrawal. This study examines the storage of canine and feline CSF samples in "TransFix®/EDTA CSF Sample Storage Tubes" (Cytomark, Buckingham, UK) for preventing leucocytes from degeneration, so that routine and flow cytometry examinations are feasible up to 3 days after sampling.

Results

After storage in TransFix® tubes, leukocytes could not be adequately stained with Türk's solution and differentiating between erythrocytes and leukocytes was cumbersome. In addition, the cell morphology could not be sufficiently assessed on cytospin preparations because of shrunken leukocytes and indistinct cell nuclei.

SUBMITTER: Meier L 

PROVIDER: S-EPMC7745459 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature