Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis.

ABSTRACT:

Background

Bone homeostasis is a tightly orchestrated process maintained by osteoblasts and osteoclasts, and a disruption of their steady-state equilibrium can lead to the occurrence of osteoporosis (OP).

Methods

We investigated the differential expression of micro (mi)RNAs in the bone tissues of a postmenopausal osteoporosis rat model induced by ovariectomy (OVX). Real-time PCR was used to verify the differentially expressed miRNAs in bone samples from OP patients and controls. The specific targets of two differentially expressed miRNAs in osteogenic or osteoclast differentiation were determined by bioinformatic prediction, and mRNA and protein detection.

Results

miR-708-5p and miR-708-3p were highly expressed in the bone tissue of OVX rats and OP patients. miR-708-5

SUBMITTER: Wang R 

PROVIDER: S-EPMC7745577 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature