4-1BB co-stimulation further enhances anti-PD-1-mediated reinvigoration of exhausted CD39⁺ CD8 T cells from primary and metastatic sites of epithelial ovarian cancers.

ABSTRACT:

Background

Responses to immunotherapy vary between different cancer types and sites. Here, we aimed to investigate features of exhaustion and activation in tumor-infiltrating CD8 T cells at both the primary and metastatic sites in epithelial ovarian cancer.

Methods

Tumor tissues and peripheral blood were obtained from 65 patients with ovarian cancer. From these samples, we isolated tumor-infiltrating lymphocytes (TILs) and peripheral blood mononuclear cells. These cells were used for immunophenotype using multicolor flow cytometry, gene expression profile using RNA sequencing and ex vivo functional restoration assays.

Results

We found that CD39⁺ CD8 TILs were enriched with tumor-specific CD8 TILs, and that the activation status of these cells was determined

SUBMITTER: Leem G 

PROVIDER: S-EPMC7745695 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature