Identification and characterization of dynamically regulated hepatitis-related genes in a concanavalin A-induced liver injury model.

ABSTRACT:

Background

Concanavalin A (ConA)-induced liver damage of mice is a well-established murine model mimicking the human autoimmune hepatitis (AIH). However, the pathogenic genes of the liver injury remain to be revealed.

Methods

Using time-series liver transcriptome, top dynamic genes were inferred from a set of segmented regression models, and cross-checked by weighted correlation network analysis (WGCNA). AIH murine models created by ConA were used to verify the in vivo effect of these genes.

Results

We identified 115 top dynamic genes, of which most were overlapped with the hub genes determined by WGCNA. The expression of several top dynamic genes including Cd63, Saa3, Slc10a1, Nrxn1, Ugt2a3, were verified in vivo. Further, Cluster determinant 63

SUBMITTER: Chen A 

PROVIDER: S-EPMC7746381 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature