Primary Extracellular Matrix Enables Long-Term Cultivation of Human Tumor Oral Mucosa Models.

ABSTRACT: 3D tumor models clearly outperform 2D cell cultures in recapitulating tissue architecture and drug response. However, their potential in understanding treatment efficacy and resistance development should be better exploited if also long-term effects of treatment could be assessed in vitro. The main disadvantages of the matrices commonly used for in vitro culture are their limited cultivation time and the low comparability with patient-specific matrix properties. Extended cultivation periods are feasible when primary human cells produce the extracellular matrix in situ. Herein, we adapted the hyalograft-3D approach from reconstructed human skin to normal and tumor oral mucosa models and compared the results to bovine collagen-based models. The hyalograft models showed s

SUBMITTER: Gronbach L 

PROVIDER: S-EPMC7746540 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature