Gigantol ameliorates CCl₄-induced liver injury via preventing activation of JNK/cPLA2/12-LOX inflammatory pathway.

ABSTRACT: Arachidonic acid (AA) signaling pathway is an important constituent of inflammatory processes. In our previous study, it was found that dihydro-stilbene gigantol relieved hepatic inflammation in mice with CCl₄-induced acute liver injury. This study aimed to investigate the involvement of arachidonate metabolic cascade in this process. Our results showed CCl₄ activated AA metabolism with the evidence of cPLA2 phosphorylation, which was dependent on the MAPK/JNK activation. Pretreatment with JNK inhibitor SU3327 or gigantol abolished the cPLA2 activation, along with the attenuation of liver damage. Besides, gigantol markedly decreased immune cells activation. Metabolomic analysis revealed that gigantol universally reversed the upregulation of major AA metabolites in inj

SUBMITTER: Xue Y 

PROVIDER: S-EPMC7746690 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature