A Rab escort protein regulates the MAPK pathway that controls filamentous growth in yeast.

ABSTRACT: MAPK pathways regulate different responses yet can share common components. Although core regulators of MAPK pathways are well known, new pathway regulators continue to be identified. Overexpression screens can uncover new roles for genes in biological processes and are well suited to identify essential genes that cannot be evaluated by gene deletion analysis. In this study, a genome-wide screen was performed to identify genes that, when overexpressed, induce a reporter (FUS1-HIS3) that responds to ERK-type pathways (Mating and filamentous growth or fMAPK) but not p38-type pathways (HOG) in yeast. Approximately 4500 plasmids overexpressing individual yeast genes were introduced into strains containing the reporter by high-throughput transformation. Candidate genes were identified by measur

SUBMITTER: Jamalzadeh S 

PROVIDER: S-EPMC7746766 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature