TRIM24 promotes stemness and invasiveness of glioblastoma cells via activating Sox2 expression.

ABSTRACT:

Background

Glioblastoma stem cells (GSCs) are a subpopulation of glioblastoma (GBM) cells that are critical for tumor invasion and treatment resistance. However, little is known about the function and mechanism of tripartite motif-containing 24 (TRIM24) in GSCs.

Methods

Immunofluorescence, flow cytometry, and western blot analyses were used to evaluate TRIM24 and cluster of differentiation (CD)133 expression profiles in GBM surgical specimens and GSC tumorspheres. Different TRIM24 expression levels in patients' tumors, as measured by both immunohistochemistry and western blot, were related to their corresponding MRI data. Wound healing, Matrigel invasion, and xenograft immunohistochemistry were conducted to determine GBM cell invasion.

Results

We identified that TRIM2

SUBMITTER: Zhang LH 

PROVIDER: S-EPMC7746937 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature