In Situ Genetically Cascaded Amplification for Imaging RNA Subcellular Locations.

ABSTRACT: In situ amplification methods, such as hybridization chain reaction, are valuable tools for mapping the spatial distribution and subcellular location of target analytes. However, the live-cell applications of these methods are still limited due to challenges in the probe delivery, degradation, and cytotoxicity. Herein, we report a novel genetically encoded in situ amplification method to noninvasively image the subcellular location of RNA targets in living cells. In our system, a fluorogenic RNA reporter, Broccoli, was split into two nonfluorescent fragments and conjugated to the end of two RNA hairpin strands. The binding of one target RNA can then trigger a cascaded hybridization between these hairpin pairs and thus activate multiple Broccoli fluorescence signals. We have s

SUBMITTER: Ren K 

PROVIDER: S-EPMC7747016 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature