The hAT-family transposable element, hopper, from Bactrocera dorsalis is a functional vector for insect germline transformation.

ABSTRACT:

Background

The hopper hAT-family transposable element isolated from the Oriental fruit fly, Bactrocera dorsalis, is distantly related to both the Drosophila hobo element and the Activator element from maize. The original 3120 bp hopper^Bd-Kah element isolated from the Kahuku wild-type strain was highly degenerate and appeared to have a mutated transposase and terminal sequences, while a second 3131 bp element, hopper^Bd-we, isolated from a white eye mutant strain had an intact transposase reading frame and terminal sequences consistent with function.

Results

The hopper^Bd-we element was tested for function by its ability to mediate germline transformation in two dipteran species other than B. dorsalis. This was achieved by creating a binary vect

SUBMITTER: Handler AM 

PROVIDER: S-EPMC7747358 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature