Differentiation of Adipose Tissue-Derived CD34+/CD31- Cells into Endothelial Cells In Vitro.

ABSTRACT: In this study, CD34⁺/CD31^- progenitor cells were isolated from the stromal vascular fraction (SVF) of adipose tissue using magnetic activated cell sorting. The endothelial differentiation capability of these cells in vitro was evaluated by culturing them in vascular endothelial growth factor (VEGF) induced medium for 14 days. Viability, proliferation, differentiation and tube formation of these cells were evaluated. Cell viability study revealed that both undifferentiated and endothelial differentiated cells remained healthy for 14 days. However, the proliferation rate was higher in undifferentiated cells compared to endothelial differentiated ones. Upregulation of endothelial characteristic genes (Von Willebrand Factor (vWF) and VE Cadherin) was observed in 2D

SUBMITTER: Forghani A 

PROVIDER: S-EPMC7747864 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature