Uncertainty of EIN2^{Ser645/Ser924} Inactivation by CTR1-Mediated Phosphorylation Reveals the Complexity of Ethylene Signaling.

ABSTRACT: ETHYLENE INSENSITIVE2 (EIN2) is a key component of ethylene signaling whose activity is inhibited upon phosphorylation of Ser⁶⁴⁵ and Ser⁹²⁴ by the Raf-like CONSTITUTIVE TRIPLE-RESPONSE 1 (CTR1) in the absence of ethylene. Ethylene prevents CTR1 activity and thus EIN2^{Ser645/Ser924} phosphorylation, and subcellular trafficking of a proteolytically cleaved EIN2 C terminus (EIN2-C) from the endoplasmic reticulum to the nucleus and processing bodies triggers ethylene signaling. Here, we report an unexpected complexity of EIN2-activated ethylene signaling. EIN2 activation in part requires ethylene in the absence of CTR1-mediated negative regulation. The ein2 mutant was complemented by the transgenes encoding EIN2, EIN2 variants with mutations that either pr

SUBMITTER: Zhang J 

PROVIDER: S-EPMC7747984 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature