Variation Patterns of NLR Clusters in Arabidopsis thaliana Genomes.

ABSTRACT: The nucleotide-binding domain and leucine-rich repeat (NLR) gene family is highly expanded in the plant lineage with extensive sequence and structure polymorphisms. To survey the landscape of NLR expansion, we mined the published long-read data generated by the resistance gene enrichment sequencing of 64 diverse Arabidopsis thaliana accessions. We found that the hot spots of massive multi-gene NLR cluster expansion did not typically span the whole cluster; instead, they were restricted to a handful of, or only one, dominant radiation(s). All sequences in such a radiation were distinct from other genes in the cluster but not from each other in the clade, making it difficult to assign trustworthy reference-based orthologies when multiple reference genes were present in the radiation.

SUBMITTER: Lee RRQ 

PROVIDER: S-EPMC7747988 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature