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The plastid genome of Herpetospermum pedunculosum (Cucurbitaceae), an endangered traditional Tibetan medicinal herbs.

ABSTRACT: Herpetospermum pedunculosum (Ser.) C. B. Clarke is an important traditional Tibetan medicinal plants in the genus of Herpetospermum, Cucurbitaceae. To better determine its phylogenetic location with respect to the other Cucurbitaceae species, the complete plastome of H. pedunculosum will be reported, which is the first species with plastid genome sequence in the genus of Herpetospermum. Its whole genome is 156,531 bp in length, consisting of a pair of inverted repeats (IRs) of 26,147 bp, one large single-copy (LSC) region of 85,878 bp, and one small single-copy (SSC) region of 18,359 bp. There are 128 genes, including 83 protein-coding genes, 36 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes in the plastome. Phylogenetic analysis based on 13 complete plastomes of Cucurbitaceae species showed sisterhood of H. pedunculosum and a clade containing Trichosanthes kirilowii and Hodgsonia macrocarpa, suggesting the close relationship between tribe Schizopeponeae and tribe Sicyoeae in the family Cucurbitaceae.

SUBMITTER: Wang C

PROVIDER: S-EPMC7748728 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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The plastid genome of Herpetospermum pedunculosum (Cucurbitaceae), an endangered traditional Tibetan medicinal herbs.

Wang Chengwang C Wang Xilong X Tseringand Tamdrin T Song Yu Y Zhu Rongjie R

Mitochondrial DNA. Part B, Resources 20200110 1

Herpetospermum pedunculosum (Ser.) C. B. Clarke is an important traditional Tibetan medicinal plants in the genus of Herpetospermum, Cucurbitaceae. To better determine its phylogenetic location with respect to the other Cucurbitaceae species, the complete plastome of H. pedunculosum will be reported, which is the first species with plastid genome sequence in the genus of Herpetospermum. Its whole genome is 156,531 bp in length, consisting of a pair of inverted repeats ...[more]

PMID: 33366618

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Project description:BackgroundHerpetospermum pedunculosum (Ser.) Baill is annual scandent herbs. They are used in the treatment of piles, inflammation of the stomach and the intestines. It can survive the extreme environment of the Tibetan Plateau (TP). However, the underlying mechanisms of this adaptation to H. pedunculosum from TP remain unclear. Here, we combined physiological and metabolomics methods to analyze H. pedunculosum response to altitude gradient differences.ResultsAt high altitude, increases in the activities of Ascorbate peroxidase (APX), Glutathione reductase (GR), Dehydroascorbate reductase (DHAR), Monodehydroascorbate reductase (MDHAR), Superoxide dismutase (SOD) have been observed in leaves. Total Glutathion content, total Ascorbate content and the ASA (ascorbic acid)/docosahexaenoic acid (DHA) ration were highly elevated from low altitude to high altitude. In addition, high altitude induces decrease of the Anthocyanidin content (ANTH) and increase of abscisic acid content (ABA). The GC-MS analyses identified of 50 metabolites from leaves of H. pedunculosum. In addition, a metabolic network was constructed based on metabolomic datasets using a weighted correlation network analysis (WGCNA) approach. The network analysis uncovered 4 distinguished metabolic modules highly associated with I, II, III and IV respectively. Furthermore, the analysis successfully classified 50 samples into seven groups: carbohydrates, amino acids, organic acids, lipid components, polyamine, secondary metabolism and others.ConclusionsIn the present study, the content of parts of amino acid components increased in samples collected at higher altitudes, and most of metabolites, including carbohydrates and organic acids were assigned to the carbon metabolic pathway comprising reductive pentose phosphate pathway, glycolysis and TCA cycle, indicating the direct relationship between adaptability and the carbon metabolic pathway and amino acids in H. pedunculosum response to high altitude. The results of this study laid the foundation of the molecular mechanism on H. pedunculosum from high altitude.

Dataset Information

The plastid genome of Herpetospermum pedunculosum (Cucurbitaceae), an endangered traditional Tibetan medicinal herbs.

Publications

The plastid genome of <i>Herpetospermum pedunculosum</i> (Cucurbitaceae), an endangered traditional Tibetan medicinal herbs.

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