Dataset Information

The complete mitochondrial DNA sequence of Yimeng scorpion (Mesobuthus martensii).

ABSTRACT: Yimeng scorpion is a specific geographical indication breed of Yimeng Mountain area in China. The complete mitochondrial genome sequence of Yimeng scorpion was determined for the first time (Accession number MN597087). It is mitochondrial genome (14,840 bp) contains 13 protein-coding genes, 21tRNA genes, 2 ribosomal RNA genes and one large non-coding region (a possible control region). Moreover, tRNA-ASP-loss was observed from the Yimeng scorpion mitochondrial genome. The mitochondrial genome sequence of the Yimeng scorpion enriches data resource for further research on genetic mechanism and classification.

SUBMITTER: Zhang YZ

PROVIDER: S-EPMC7748737 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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The complete mitochondrial DNA sequence of Yimeng scorpion (<i>Mesobuthus martensii</i>).

Zhang Yan-Zhen YZ Ji Yan-Qing YQ Kang Da-Cheng DC Liu Ling-Xiao LX Liu Yun-Guo YG

Mitochondrial DNA. Part B, Resources 20191218 1

Yimeng scorpion is a specific geographical indication breed of Yimeng Mountain area in China. The complete mitochondrial genome sequence of Yimeng scorpion was determined for the first time (Accession number MN597087). It is mitochondrial genome (14,840 bp) contains 13 protein-coding genes, 21tRNA genes, 2 ribosomal RNA genes and one large non-coding region (a possible control region). Moreover, tRNA-ASP-loss was observed from the Yimeng scorpion mitochondrial genome. The mitochondrial genome se ...[more]

PMID: 33366545

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Project description:α-Scorpion toxins constitute a family of peptide modulators that induce a prolongation of the action potential of excitable cells by inhibiting voltage-gated sodium channel inactivation. Although they all adopt a conserved structural scaffold, the potency and phylogentic preference of these toxins largely vary, which render them an intriguing model for studying evolutionary diversification among family members. Here, we report molecular characterization of a new multigene family of α-toxins comprising 13 members (named MeuNaTxα-1 to MeuNaTxα-13) from the scorpion Mesobuthus eupeus. Of them, five native toxins (MeuNaTxα-1 to -5) were purified to homogeneity from the venom and the solution structure of MeuNaTxα-5 was solved by nuclear magnetic resonance. A systematic functional evaluation of MeuNaTxα-1, -2, -4, and -5 was conducted by two-electrode voltage-clamp recordings on seven cloned mammalian voltage-gated sodium channels (Na(v)1.2 to Na(v)1.8) and the insect counterpart DmNa(v)1 expressed in Xenopus oocytes. Results show that all these four peptides slow inactivation of DmNa(v)1 and are inactive on Na(v)1.8 at micromolar concentrations. However, they exhibit differential specificity for the other six channel isoforms (Na(v)1.2 to Na(v)1.7), in which MeuNaTxα-4 shows no activity on these isoforms and thus represents the first Mesobuthus-derived insect-selective α-toxin identified so far with a half maximal effective concentration of 130 ± 2 nm on DmNa(v)1 and a half maximal lethal dose of about 200 pmol g(-1) on the insect Musca domestica; MeuNaTxα-2 only affects Na(v)1.4; MeuNaTxα-1 and MeuNaTxα-5 have a wider range of channel spectrum, the former active on Na(v)1.2, Na(v)1.3, Na(v)1.6, and Na(v)1.7, whereas the latter acting on Na(v)1.3-Na(v)1.7. Remarkably, MeuNaTxα-4 and MeuNaTxα-5 are two nearly identical peptides differing by only one point mutation at site 50 (A50V) but exhibit rather different channel subtype selectivity, highlighting a switch role of this site in altering the target specificity. By the maximum likelihood models of codon substitution, we detected nine positively selected sites (PSSs) that could be involved in functional diversification of Mesobuthus α-toxins. The PSSs include site 50 and other seven sites located in functional surfaces of α-toxins. This work represents the first thorough investigation of evolutionary diversification of α-toxins derived from a specific scorpion lineage from the perspectives of sequence, structure, function, and evolution.

Dataset Information

The complete mitochondrial DNA sequence of Yimeng scorpion (Mesobuthus martensii).

Publications

The complete mitochondrial DNA sequence of Yimeng scorpion (<i>Mesobuthus martensii</i>).

Similar Datasets

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