Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sequencing and analysis of the complete mitochondrial genome of Mansonia uniformis (Dipera: Culicidae).

ABSTRACT: In this study, we sequenced and analyzed the complete mitochondrial genome of Mansonia uniformis, and this is the first report on the genus Mansonia. The circular mitogenome is 15,603 bp long and contains 13 protein-coding genes (PCGs), 22 tRNA genes, 2 ribosomal RNA genes, and a A + T-rich control region. Most PCGs start with ATN codons, and end with TAA, except for COX1 starting with TCG codons and COX2 ending with a single thymine stop codon. The phylogenetic tree based on the COX1 gene showed that M. uniformis formed a monophyletic clade, sister to other seven genus from the subfamily Culicinae.

SUBMITTER: Tian J

PROVIDER: S-EPMC7748744 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

ACCESS DATA

Json Xml

Publications

Sequencing and analysis of the complete mitochondrial genome of Mansonia uniformis (Dipera: Culicidae).

Tian Junhua J Yu Bin B Shi Xianfeng X Liang Huan H Wang Dehuan D Ge Mihong M

Mitochondrial DNA. Part B, Resources 20200110 1

In this study, we sequenced and analyzed the complete mitochondrial genome of Mansonia uniformis, and this is the first report on the genus Mansonia. The circular mitogenome is 15,603 bp long and contains 13 protein-coding genes (PCGs), 22 tRNA genes, 2 ribosomal RNA genes, and a A + T-rich control region. Most PCGs start with ATN codons, and end with TAA, except for COX1 starting with TCG codons and COX2 ending with a single thymine stop codon. The phylogenetic tree based o ...[more]

PMID: 33366619

Similar Datasets

Sequencing and analysis of the complete mitochondrial genome in Anopheles sinensis (Diptera: Culicidae).

Project description:BackgroundAnopheles sinensis (Diptera: Culicidae) is a primary vector of Plasmodium vivax and Brugia malayi in most regions of China. In addition, its phylogenetic relationship with the cryptic species of the Hyrcanus Group is complex and remains unresolved. Mitochondrial genome sequences are widely used as molecular markers for phylogenetic studies of mosquito species complexes, of which mitochondrial genome data of An. sinensis is not available.MethodsAn. sinensis samples was collected from Shandong, China, and identified by molecular marker. Genomic DNA was extracted, followed by the Illumina sequencing. Two complete mitochondrial genomes were assembled and annotated using the mitochondrial genome of An. gambiae as reference. The mitochondrial genomes sequences of the 28 known Anopheles species were aligned and reconstructed phylogenetic tree by Maximum Likelihood (ML) method.FindingsThe length of complete mitochondrial genomes of An. sinensis was 15,076 bp and 15,138 bp, consisting of 13 protein-coding genes, 22 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes, and an AT-rich control region. As in other insects, most mitochondrial genes are encoded on the J strand, except for ND5, ND4, ND4L, ND1, two rRNA and eight tRNA genes, which are encoded on the N strand. The bootstrap value was set as 1000 in ML analyses. The topologies restored phylogenetic affinity within subfamily Anophelinae. The ML tree showed four major clades, corresponding to the subgenera Cellia, Anopheles, Nyssorhynchus and Kerteszia of the genus Anopheles.ConclusionsThe complete mitochondrial genomes of An. sinensis were obtained. The number, order and transcription direction of An. sinensis mitochondrial genes were the same as in other species of family Culicidae.

| S-EPMC5625653 | biostudies-literature

Sequencing and analysis of the complete mitochondrial genome of Culex gelidus (Diptera: Culicidae).

Project description:In this study, we sequenced and analyzed the complete mitochondrial genome of Culex gelidus. The mitogenome is 15,600 bp long, and contains 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a control region. The gene order and composition are identical with those mitogenomes reported in other mosquito species. The whole base composition is A (39.8%), T (39.2%), G (8.8%), and C (12.2%). The PCGs have the initiation codon ATN except for COI with a TCG, and possess the complete termination codon TAA or incomplete T. The phylogenetic analysis of known Culex mitogenome sequences was carried out based on the nucleotide sequences of 13 PCGs, and the result showed that Cx. P. pipiens, Cx. P. pallens, and Cx. p. quinquefasciatus were claded into the Pipiens Complex in the Pipiens Group, and Cx. gelidus might be inappropriate to be classified into the Pipiens Group. Whether the mosquito species group with Cx. gelidus as type should be regarded as a Gelidus Group or Subgroup and its taxonomic position need to be elucidated with more molecular data.

| S-EPMC7800811 | biostudies-literature

Sequencing and analysis of the complete mitochondrial genome in Anopheles culicifacies species B (Diptera: Culicidae).

Project description:The complete mitochondrial genome sequence of Anopheles culicifacial species B was sequenced in this study. The length of the mitochondrial genome is 15 330 bp, which contains 13 protein-coding genes (PCGs), 22 tRNA genes, two rRNA genes, and a non-coding control region. The gene order and the gene composition are consistent with those previously reported for other mosquito species. The initiation codon of the PCGs complies with the ATN rule except for COI using TCG and ND5 using GTG as a start codon, and the termination codon is TAA or imcomplete, an only T. The total base composition is 40.4% A, 38.1% T, 12.4% C, and 9.1% G. The phylogenetic tree based on the sequences of 13 protein-coding genes showed that these species were classified into two clades, corresponding to the subgenus Cellia and subgenus Nyssorhynchus. An. culicifacies species B of Myzomyia Series was clustered with An. gambiae of Pyretophorus Series with a high bootstrap value of 100%. The complete mitogenome data can provide a basis for molecular identification and phylogenetic studies of mosquito species.

| S-EPMC4902849 | biostudies-literature

Sequencing and analysis of the complete mitochondrial genome of Culex tritaeniorhynchus (Dipera: Culicidae) in China.

Project description:The complete sequence of the mitochondrial genome of the Culex tritaeniorhynchus (Diptera: Culicidae) is presented using traditional Sanger sequencing. Its mitogenome are 15,123 bp in length, consisting of 13 protein-coding genes, 2 ribosomal RNA genes (rRNA) and 22 transfer RNA (tRNA) genes. The percent of A + T of the mitogenome is 77.73%. Most mitochondrial genes are encoded on the heavy strand, except for NAD5, NaD4, NAD4L, NAD1, two rRNA and nine tRNA genes, which are encoded on the light strand. The results of phylogenetic analyzes showed that the Cx. tritaeniorhynchus has close relationship with the species of genus Culex.

| S-EPMC7800139 | biostudies-literature

DNA Barcodes of Mansonia (Mansonia) Blanchard, 1901 (Diptera, Culicidae).

Project description:Females of the genus Mansonia feed on the blood of humans, livestock, and other vertebrates to develop their eggs. The females' biting behavior may cause severe disturbance to blood hosts, with a negative impact on public health and economics. Certain species have been identified as potential or effective disease vectors. The accurate species identification of field-collected specimens is of paramount importance for the success of monitoring and control strategies. Mansonia (Mansonia) morphological species boundaries are blurred by patterns of intraspecific heteromorphism and interspecific isomorphism. DNA barcodes can help to solve taxonomic controversies, especially if combined with other molecular tools. We used cytochrome c oxidase subunit I (COI) gene 5' end (DNA barcode) sequences to identify 327 field-collected specimens of Mansonia (Mansonia) spp. The sampling encompassed males and females collected from three Brazilian regions and previously assigned to species based on their morphological characteristics. Eleven GenBank and BOLD sequences were added to the DNA barcode analyses. Initial morphospecies assignments were mostly corroborated by the results of five clustering methods based on Kimura two-parameter distance and maximum likelihood phylogeny. Five to eight molecular operational taxonomic units may represent taxonomically unknown species. The first DNA barcode records for Mansonia fonsecai, Mansonia iguassuensis, and Mansonia pseudotitillans are presented.

| S-EPMC10298705 | biostudies-literature

Complete Genome Sequences of Two Bacteroides uniformis Bacteriophages.

Project description:Here, we describe the isolation and genomic annotation of two novel siphovirus species of bacteriophages that infect Bacteroides uniformis: Bacteroides phage EMB1 and Bacteroides phage EMB2. EMB1 has a 34,204-bp genome with 48 coding sequences, and EMB2 has a 34,008-bp genome with 47 coding sequences.

| S-EPMC9584325 | biostudies-literature

The complete mitochondrial genome of Culex hutchinsoni (Diptera: Culicidae) and its phylogenetic analysis.

Project description:The complete mitochondrial genome of Culex hutchinsoni Barraud, 1924 was reported with 15,586 bp long. It contained 37 annotated genes and the control region, which is consistent with other mosquitoes. Most PCGs used typical ATN as start codons, while some special start codons were also used (i.e. TCG for COX1, TTG for ND1, GTG for ND5). Most PCGs terminated with TAA as well as some PCGs (COX1, COX2, COX3, ND3, ND4 and CYTB) terminated with TA-tRNA or T-tRNA. Cx. (Lophoceraomyia) was recovered as the sister group to Cx. (Culex), while Cx. hutchinsoni was recovered within the species group of Cx. pipiens.

| S-EPMC11610350 | biostudies-literature

Complete mitochondrial genome of Andrias davidianus (Caudata: cryptobrachidae): sequencing and analysis.

Project description:In this study, the total mitochondrial genome sequence of Andrias davidianus (Caudata: Cryptobrachidae) was determined. The genome is 16,519 bases in length. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 2 non-coding regions. These results will contribute to the natural resources conservation and species identification Of A. davidianus.

| S-EPMC7799949 | biostudies-literature

Sequencing and phylogenetic analysis of the Pyrgilauda ruficollis (Aves, Passeridae) complete mitochondrial genome.

Project description:In this study, both long PCR and conserved primers walking sequencing methods were used to determine the complete sequence of the of Pyrgilauda ruficollis mitochondrial genome (KC836121). The results showed that the complete mitochondrial genome of P. ruficollis is 1 6909 bp in length with 55.0% A+T content, harboring the typical 37 genes. The mitogenome had the same gene order with that of Podoces hendersoni. All protein coding genes started with ATG codon, except ND3 with GTG. For the stop codon usage, most genes terminate with codons TAA or TAG, but ND5 terminated with AGA, while ND1 and COI genes with AGG, and both the genes COⅢ and ND4 have an incomplete termination codon (T). The secondary structures of 22 tRNA genes were also predicted, showing that all tRNAs can form typical clover-leaf secondary structures, except for the tRNA(Ser) (AGN) which loses the DHU arm, while tRNA(Phe) harbor an extra nucleotide inserted in the TψC arm. The predicted secondary structures of 12S rRNA and 16S rRNA exhibit 47 helices in 4 domains and 60 helices in 6 domains respectively. The control region of P. ruficollis with the length of 1 305 bp was located between tRNA(Glu) and tRNA(Phe), and typical domains of which could be found as other bird groups. Using the data from 13 mitochondrial protein-coding genes, results of a final phylogenetic analysis strongly supports the traditional view that P. ruficollis is closely related with Passeridae and Fringillidae.

| S-EPMC5042932 | biostudies-literature

Sequencing and analysis of the complete mitochondrial genome of Hippopus porcellanus.

Project description:In this study, the complete mitochondrial genome of Hippopus porcellanus was reported. The whole mitochondrial genome was 21,565bp in length with a typical mitochondrial genomic structure including 13 protein-coding genes, 23 transfer RNA genes, 2 ribosomal RNA genes and 1 control region (D-loop). Mitogenome base composition was biased toward A + T content, at 60.3%. A phylogenetic tree based on complete mitogenome sequences revealed that, H. porcellanus is closely related to H. hippopus, both of which belong to the genus Hippopus.

| S-EPMC8317960 | biostudies-literature