Improved High-Throughput Sequencing of the Human Oral Microbiome: From Illumina to PacBio.

ABSTRACT:

Background

A comprehensive understanding of the commensal microflora and its relation to health is essential for preventing and combating diseases. The aim of this study was to examine the structure of the oral microbiome by using different sequencing technologies. Material and Methods. Five preschool children with no symptoms of oral and systemic diseases were recruited. Samples of saliva were collected. A 468 bp insert size library was constructed on the MiSeq platform and then subjected to 300 bp paired-end sequencing. Libraries with longer insert sizes, including a full-length 16S rDNA gene, were sequenced on the PacBio RS II platform.

Results

A total of 122.6 Mb of raw data, including 244,967 high-quality sequences, were generated by the MiSeq platform, while 134

SUBMITTER: Zhang J 

PROVIDER: S-EPMC7748900 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature