Creating Red Light-Switchable Protein Dimerization Systems as Genetically Encoded Actuators with High Specificity.

ABSTRACT: Protein dimerization systems controlled by red light with increased tissue penetration depth are a highly needed tool for clinical applications such as cell and gene therapies. However, mammalian applications of existing red light-induced dimerization systems are hampered by limitations of their two components: a photosensory protein (or photoreceptor) which often requires a mammalian exogenous chromophore and a naturally occurring photoreceptor binding protein typically having a complex structure and nonideal binding properties. Here, we introduce an efficient, generalizable method (COMBINES-LID) for creating highly specific, reversible light-induced heterodimerization systems independent of any existing binders to a photoreceptor. It involves a two-step binder screen (phage display and y

SUBMITTER: Huang Z 

PROVIDER: S-EPMC7749050 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature