An anchoring molecule increases intravitreal retention of antibody-based therapeutics used in the treatment of ocular diseases.

ABSTRACT: Intravitreal delivery of antibody-based therapeutics has revolutionized the treatment of intraocular vascular diseases involving the retina and choroid. Unfortunately, limited durability requires frequent retreatment placing an enormous burden on patients. We sought to solve this problem with a novel approach that uses an anchoring molecule characterized by two key molecular properties: (1) non-covalent binding to an antibody-based therapeutic, and (2) retention in the vitreous cavity. As an initial proof-of-principle, we chose an anchoring molecule composed of agarose microbeads functionalized with an Fc-binding domain. Bevacizumab was chosen as the antibody-based therapeutic. In vitro experiments demonstrated that bevacizumab was maximally bound to this anchoring molecule within 1 h, and

SUBMITTER: Gao Q 

PROVIDER: S-EPMC7749058 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature