Transcriptional profiles of human islet and exocrine endothelial cells in subjects with or without impaired glucose metabolism.

ABSTRACT: In experimental studies, pancreatic islet microvasculature is essential for islet endocrine function and mass, and islet vascular morphology is altered in diabetic subjects. Even so, almost no information is available concerning human islet microvascular endothelial cell (MVEC) physiology and gene expression. In this study, islets and exocrine pancreatic tissue were acquired from organ donors with normoglycemia or impaired glucose metabolism (IGM) immediately after islet isolation. Following single-cell dissociation, primary islet- and exocrine MVECs were obtained through fluorescence-activated cell sorting (FACS) and transcriptional profiles were generated using AmpliSeq. Multiple gene sets involved in general vascular development and extracellular matrix remodeling were enriched in islet

SUBMITTER: Jonsson A 

PROVIDER: S-EPMC7749106 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature