Generation and initial characterization of novel tumour organoid models to study human pancreatic cancer-induced cachexia.

ABSTRACT:

Background

The majority of patients with pancreatic cancer develops cachexia. The mechanisms underlying cancer cachexia development and progression remain elusive, although tumour-derived factors are considered to play a major role. Pancreatic tumour organoids are in vitro three-dimensional organ-like structures that retain many pathophysiological characteristics of the in vivo tumour. We aimed to establish a pancreatic tumour organoid biobank from well-phenotyped cachectic and non-cachectic patients to enable identification of tumour-derived factors driving cancer cachexia.

Methods

Organoids were generated from tumour tissue of eight pancreatic cancer patients. A comprehensive pre-operative patient assessment of cachexia-related parameters including nutritional status, phys

SUBMITTER: Vaes RDW 

PROVIDER: S-EPMC7749546 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature