A plausible involvement of plasmalemmal voltage-dependent anion channel 1 in the neurotoxicity of 15-deoxy-Δ^12,14 -prostaglandin J₂.

ABSTRACT:

Introduction

15-deoxy-Δ^12,14 -prostaglandin J₂ (15d-PGJ₂ ) causes neuronal apoptosis independently of its nuclear receptor, peroxysome-proliferator activated receptor γ. Its membrane receptor, chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTH2), did not also mediate the neurotoxicity of 15d-PGJ₂ . In the present study, we ascertained whether membrane targets beside CRTH2 were involved in the neurotoxicity of 15d-PGJ₂ .

Methods

Neuronal membrane targets for 15d-PGJ₂ were separated by two-dimensional electrophoresis, identified by proteomic approach. Their localizations were detected by microscopic immunofluorescence study. Cell viability and apoptosis was evaluated by MTT-reducing activi

SUBMITTER: Koma H 

PROVIDER: S-EPMC7749624 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature