Combination of circulating miR-145-5p/miR-191-5p as biomarker for breast cancer detection.

ABSTRACT:

Background

Breast cancer (BC) is the most common cancer among women worldwide. At present, there is a need to search for new, accurate, reliable, minimally invasive and cheap biomarkers in addition to existing methods for the diagnosis and prognosis of BC. The main goal of this study was to test the diagnostic value of six circulating miRNAs in Kazakh women.

Materials and methods

TaqMan-based miRNA profiling was conducted using plasma specimens from 35 BC women patients and 33 healthy women samples (control group).

Results

The level of all seven miRNAs (including endogenous control) normalized by synthetic cel-miR-39 were significantly elevated in the group of BC patients. Normalization using miR-222-3p as endogenous control reduced differences in level of miRNAs betw

SUBMITTER: Ashirbekov Y 

PROVIDER: S-EPMC7749656 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature