Characterization of DNA methylation in Malawian Mycobacterium tuberculosis clinical isolates.

ABSTRACT:

Background

Although Mycobacterium tuberculosis (Mtb) strains exhibit genomic homology of >99%, there is considerable variation in the phenotype. The underlying mechanisms of phenotypic heterogeneity in Mtb are not well understood but epigenetic variation is thought to contribute. At present the methylome of Mtb has not been completely characterized.

Methods

We completed methylomes of 18 Mycobacterium tuberculosis (Mtb) clinical isolates from Malawi representing the largest number of Mtb genomes to be completed in a single study using Single Molecule Real Time (SMRT) sequencing to date.

Results

We replicate and confirm four methylation disrupting mutations in 4 lineages of Mtb. For the first time we report complete loss

SUBMITTER: Ndhlovu V 

PROVIDER: S-EPMC7749660 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature