Adaptive optics improves multiphoton super-resolution imaging.

ABSTRACT: We improve multiphoton structured illumination microscopy using a nonlinear guide star to determine optical aberrations and a deformable mirror to correct them. We demonstrate our method on bead phantoms, cells in collagen gels, nematode larvae and embryos, Drosophila brain, and zebrafish embryos. Peak intensity is increased (up to 40-fold) and resolution recovered (up to 176 ± 10 nm laterally, 729 ± 39 nm axially) at depths ?250 ?m from the coverslip surface.

SUBMITTER: Zheng W 

PROVIDER: S-EPMC7749710 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature