Whole body deletion of Gpr68 does not change hematopoietic stem cell function.

ABSTRACT: G protein-coupled receptor 68 (GPR68) responds to extracellular protons, thus called the proton-sensing G protein-coupled receptor (GPCR), leading to activation of the phospholipase C-β (PLCβ)/calcium (Ca²⁺) pathway or the adenylyl cyclase (AC)/cyclic AMP (cAMP) pathway. We recently found that whole body deletion of Gpr68 (Gpr68^-/- mice) reduced the number of B lymphocytes with age and during hematopoietic regeneration, such as in response to fluorouracil (5-FU) administration. This prompted us to characterize the hematopoietic stem cell (HSC) phenotype in Gpr68^-/- mice. Despite high level of Gpr68 protein expression on HSC in bone marrow (BM), the pool size of HSC was unaltered in Gpr68^-/- mice either under steady state or upon stress, including

SUBMITTER: He X 

PROVIDER: S-EPMC7749853 | biostudies-literature | 2020 Aug

REPOSITORIES: biostudies-literature