Oxidation of protein disulfide bonds by singlet oxygen gives rise to glutathionylated proteins.

ABSTRACT: Disulfide bonds play a key function in determining the structure of proteins, and are the most strongly conserved compositional feature across proteomes. They are particularly common in extracellular environments, such as the extracellular matrix and plasma, and in proteins that have structural (e.g. matrix) or binding functions (e.g. receptors). Recent data indicate that disulfides vary markedly with regard to their rate of reaction with two-electron oxidants (e.g. HOCl, ONOOH), with some species being rapidly and readily oxidized. These reactions yielding thiosulfinates that can react further with a thiol to give thiolated products (e.g. glutathionylated proteins with glutathione, GSH). Here we show that these 'oxidant-mediated thiol-disulfide exchange reactions' also occur during photo-

SUBMITTER: Jiang S 

PROVIDER: S-EPMC7750407 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature