Discovery of Novel Biosynthetic Gene Cluster Diversity From a Soil Metagenomic Library.

ABSTRACT: Soil microorganisms historically have been a rich resource for natural product discovery, yet the majority of these microbes remain uncultivated and their biosynthetic capacity is left underexplored. To identify the biosynthetic potential of soil microorganisms using a culture-independent approach, we constructed a large-insert metagenomic library in Escherichia coli from a topsoil sampled from the Cullars Rotation (Auburn, AL, United States), a long-term crop rotation experiment. Library clones were screened for biosynthetic gene clusters (BGCs) using either PCR or a NGS (next generation sequencing) multiplexed pooling strategy, coupled with bioinformatic analysis to identify contigs associated with each metagenomic clone. A total of 1,015 BGCs were detected from 19,200 clones, ide

SUBMITTER: Santana-Pereira ALR 

PROVIDER: S-EPMC7750434 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature