Fundamentally different repetitive element composition of sex chromosomes in Rumex acetosa.

ABSTRACT:

Background and aims

Dioecious species with well-established sex chromosomes are rare in the plant kingdom. Most sex chromosomes increase in size but no comprehensive analysis of the kind of sequences that drive this expansion has been presented. Here we analyse sex chromosome structure in common sorrel (Rumex acetosa), a dioecious plant with XY1Y2 sex determination, and we provide the first chromosome-specific repeatome analysis for a plant species possessing sex chromosomes.

Methods

We flow-sorted and separately sequenced sex chromosomes and autosomes in R. acetosa using the two-dimensional fluorescence in situ hybridization in suspension (FISHIS) method and Illumina sequencing. We identified and quantified individual repeats using RepeatExplorer, Tandem Repeat Finder and t

SUBMITTER: Jesionek W 

PROVIDER: S-EPMC7750719 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature