Concerted and birth-and-death evolution of 26S ribosomal DNA in Camellia L.

ABSTRACT:

Background and aims

The ribosomal DNA (rDNA) gene family, encoding ribosomal RNA (rRNA), has long been regarded as an archetypal example illustrating the model of concerted evolution. However, controversy is arising, as rDNA in many eukaryotic species has been proved to be polymorphic. Here, a metagenomic strategy was applied to detect the intragenomic polymorphism as well as the evolutionary patterns of 26S rDNA across the genus Camellia.

Methods

Degenerate primer pairs were designed to amplify the 26S rDNA fragments from different Camellia species. The amplicons were then paired-end sequenced on the Illumina MiSeq platform.

Key results

An extremely high level of rDNA polymorphism existed universally in Camellia. However, functional rDNA was still the major component

SUBMITTER: Zhang M 

PROVIDER: S-EPMC7750723 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature