Genetic Mutations That Drive Evolutionary Rescue to Lethal Temperature in Escherichia coli.

ABSTRACT: Evolutionary rescue occurs when adaptation restores population growth against a lethal stressor. Here, we studied evolutionary rescue by conducting experiments with Escherichia coli at the lethal temperature of 43.0 °C, to determine the adaptive mutations that drive rescue and to investigate their effects on fitness and gene expression. From hundreds of populations, we observed that ∼9% were rescued by genetic adaptations. We sequenced 26 populations and identified 29 distinct mutations. Of these populations, 21 had a mutation in the hslVU or rpoBC operon, suggesting that mutations in either operon could drive rescue. We isolated seven strains of E. coli carrying a putative rescue mutation in either the hslVU or rpoBC operon to investigate the mutations' effects. The single rescue mutation

SUBMITTER: Batarseh TN 

PROVIDER: S-EPMC7750951 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature