Predicting substitutions to modulate disorder and stability in coiled-coils.

ABSTRACT:

Background

Coiled-coils are described as stable structural motifs, where two or more helices wind around each other. However, coiled-coils are associated with local mobility and intrinsic disorder. Intrinsically disordered regions in proteins are characterized by lack of stable secondary and tertiary structure under physiological conditions in vitro. They are increasingly recognized as important for protein function. However, characterizing their behaviour in solution and determining precisely the extent of disorder of a protein region remains challenging, both experimentally and computationally.

Results

In this work, we propose a computational framework to quantify the extent of disorder within a coiled-coil in solution and to help design substitutions modulating such disor

SUBMITTER: Karami Y 

PROVIDER: S-EPMC7751101 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature