Minimum dietary methionine requirements in Miniature Dachshund, Beagle, and Labrador Retriever adult dogs using the indicator amino acid oxidation technique.

ABSTRACT: The objective of this study was to determine the minimum requirement (MR) for methionine (Met), when cyst(e)ine (Cys) is provided in excess, in adult dogs of three different breed sizes using the indicator amino acid (AA) oxidation (IAAO) technique. In total, 12 adult dogs were used: 1 neutered and 3 spayed Miniature Dachshunds (4.8 ± 0.4 kg body weight [BW], mean ± SD), 4 spayed Beagles (9.5 ± 0.7 kg BW, mean ± SD), and 4 neutered Labrador Retrievers (31.8 ± 1.7 kg BW, mean ± SD). A deficient Met basal diet with excess Cys was formulated. Dogs were fed the basal diet randomly supplemented with different Met-Alanine (Ala) solutions to achieve final Met concentrations in experimental diets of 0.21%, 0.26%, 0.31%, 0.36%, 0.41%, 0.46%, and 0.66% (as-fed basis). After 2 d of adaptation to the

SUBMITTER: Mansilla WD 

PROVIDER: S-EPMC7751151 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature