Expression and Importance of TMED2 in Multiple Myeloma Cells.

ABSTRACT:

Objective

TMED2 is a member of the transmembrane emp24 domain (Tmed)/p24 protein family, which is significantly upregulated in breast cancer, ovarian cancer and other tumour tissues. The purpose of this study was to investigate the expression of TMED2 in MM cell lines and its effect on the biological behaviour of MM cell lines.

Methods

Real-time quantitative PCR (RT-qPCR) was used to detect the expression of TMED2 in MM cell lines, including MM.1S and RPMI 8226 cells, and lentivirus vector-mediated TMED2 gene silencing was used to further study the effect of the downregulation of TMED2 expression on cell viability, the cell cycle, and apoptosis.

Results

Based on the RT-qPCR results, the expression of the TMED2 mRNA was increased in the MM cell lin

SUBMITTER: Ge X 

PROVIDER: S-EPMC7751311 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature