Comparison of long-read methods for sequencing and assembly of a plant genome.

ABSTRACT:

Background

Sequencing technologies have advanced to the point where it is possible to generate high-accuracy, haplotype-resolved, chromosome-scale assemblies. Several long-read sequencing technologies are available, and a growing number of algorithms have been developed to assemble the reads generated by those technologies. When starting a new genome project, it is therefore challenging to select the most cost-effective sequencing technology, as well as the most appropriate software for assembly and polishing. It is thus important to benchmark different approaches applied to the same sample.

Results

Here, we report a comparison of 3 long-read sequencing technologies applied to the de novo assembly of a plant genome, Macadamia jansenii. We have generated sequencing data using

SUBMITTER: Murigneux V 

PROVIDER: S-EPMC7751402 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature