Molecular Characterization of Carbapenem/Colistin-Resistant Acinetobacter baumannii Clinical Isolates from Egypt by Whole-Genome Sequencing.

ABSTRACT:

Purpose

The rise of carbapenem-resistant A. baumannii (CRAB) is considered a public health problem limiting the treatment options. Our current work studied the emergence and mechanisms of colistin-resistance among CRAB isolates in Egypt.

Materials and methods

Seventeen clinically recovered A. baumannii were identified and screened for their antimicrobial susceptibilities using VITEK-2 system. Colistin susceptibility was evaluated using broth microdilution, and characterization of carbapenem/colistin resistance determinants was performed using whole-genome sequencing (Illumina MiSeq).

Results

About 52.9% (9/17) were colistin-resistant. PCR results revealed that all isolates carried bla _{OXA-51-like genes}, bla _OXA-23-like

SUBMITTER: Fam NS 

PROVIDER: S-EPMC7751577 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature