Membrane Dynamics and Organization of the Phagocyte NADPH Oxidase in PLB-985 Cells.

ABSTRACT: Neutrophils are the first cells recruited at the site of infections, where they phagocytose the pathogens. Inside the phagosome, pathogens are killed by proteolytic enzymes that are delivered to the phagosome following granule fusion, and by reactive oxygen species (ROS) produced by the NADPH oxidase. The NADPH oxidase complex comprises membrane proteins (NOX2 and p22^phox), cytoplasmic subunits (p67^phox, p47^phox, and p40^phox) and the small GTPase Rac. These subunits assemble at the phagosomal membrane upon phagocytosis. In resting neutrophils the catalytic subunit NOX2 is mainly present at the plasma membrane and in the specific granules. We show here that NOX2 is also present in early and recycling endosomes in human neutrophils and in the neutr

SUBMITTER: Joly J 

PROVIDER: S-EPMC7751761 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature