Inhibition of mitochondrial fission and iNOS in the dorsal vagal complex protects from overeating and weight gain.

ABSTRACT:

Objectives

The dorsal vagal complex (DVC) senses insulin and controls glucose homeostasis, feeding behaviour and body weight. Three-days of high-fat diet (HFD) in rats are sufficient to induce insulin resistance in the DVC and impair its ability to regulate feeding behaviour. HFD-feeding is associated with increased dynamin-related protein 1 (Drp1)-dependent mitochondrial fission in the DVC. We investigated the effects that altered Drp1 activity in the DVC has on feeding behaviour. Additionally, we aimed to uncover the molecular events and the neuronal cell populations associated with DVC insulin sensing and resistance.

Methods

Eight-week-old male Sprague Dawley rats received DVC stereotactic surgery for brain infusion to facilitate the localised administration of insulin or

SUBMITTER: Patel B 

PROVIDER: S-EPMC7753200 | biostudies-literature | 2021 Jan

REPOSITORIES: biostudies-literature