Production of Propene from n-Butanol: A Three-Step Cascade Utilizing the Cytochrome P450 Fatty Acid Decarboxylase OleT_JE.

ABSTRACT: Propene is one of the most important starting materials in the chemical industry. Herein, we report an enzymatic cascade reaction for the biocatalytic production of propene starting from n-butanol, thus offering a biobased production from glucose. In order to create an efficient system, we faced the issue of an optimal cofactor supply for the fatty acid decarboxylase OleT_JE , which is said to be driven by either NAD(P)H or H₂ O₂ . In the first system, we used an alcohol and aldehyde dehydrogenase coupled to OleT_JE by the electron-transfer complex putidaredoxin reductase/putidaredoxin, allowing regeneration of the NAD⁺ cofactor. With the second system, we intended full oxidation of n-butanol to butyric acid, generating one equivalent of

SUBMITTER: Bauer D 

PROVIDER: S-EPMC7754297 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature