Generation and reactivity analysis of human recombinant monoclonal antibodies directed against epitopes on HLA-DR.

ABSTRACT: In kidney transplantation, eplet mismatches between donor and recipient have been associated with de novo donor-specific antibody development. Eplets are theoretically defined configurations of polymorphic amino acids and require experimental verification to establish whether they can be bound by alloantibodies. Human HLA-specific monoclonal antibodies (mAbs) have been instrumental for this purpose but are largely lacking for HLA class II. In this study, we isolated single HLA-DR-specific memory B cells from peripheral blood of immunized individuals (n = 3) using HLA class II tetramers to generate recombinant human HLA-DR antigen-reactive mAbs (n = 5). Comparison of the amino acid composition of the reactive HLA alleles in relation to the antibody reactivity patterns led to identification

SUBMITTER: Kramer CSM 

PROVIDER: S-EPMC7754395 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature