Alport syndrome: Proteomic analysis identifies early molecular pathway alterations in Col4a3 knock out mice.

ABSTRACT:

Aim

Alport syndrome (AS) is the second most common hereditary kidney disease caused by mutations in collagen IV genes. Patients present with microhaematuria that progressively leads to proteinuria and end stage renal disease. Currently, no specific treatment exists for AS. Using mass spectrometry based proteomics, we aimed to detect early alterations in molecular pathways implicated in AS before the stage of overt proteinuria, which could be amenable to therapeutic intervention.

Methods

Kidneys were harvested from male Col4a3^-/- knock out and sex and age-matched Col4a3^+/+ wild-type mice at 4 weeks of age. Purified peptides were separated by liquid chromatography and analysed by high resolution mass spectrometry. The Cytoscape bioinformatics tool was use

SUBMITTER: Nicolaou O 

PROVIDER: S-EPMC7754404 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature