The alarmone (p)ppGpp confers tolerance to oxidative stress during the stationary phase by maintenance of redox and iron homeostasis in Staphylococcus aureus.

ABSTRACT: Slow growing stationary phase bacteria are often tolerant to multiple stressors and antimicrobials. Here, we show that the pathogen Staphylococcus aureus develops a non-specific tolerance towards oxidative stress during the stationary phase, which is mediated by the nucleotide second messenger (p)ppGpp. The (p)ppGpp⁰ mutant was highly susceptible to HOCl stress during the stationary phase. Transcriptome analysis of the (p)ppGpp⁰ mutant revealed an increased expression of the PerR, SigB, QsrR, CtsR and HrcA regulons during the stationary phase, indicating an oxidative stress response. The (p)ppGpp⁰ mutant showed a slight oxidative shift in the bacillithiol (BSH) redox potential (E_BSH) and an impaired H₂O₂ detoxification due t

SUBMITTER: Fritsch VN 

PROVIDER: S-EPMC7754856 | biostudies-literature | 2020 Dec

REPOSITORIES: biostudies-literature